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Chicken Tumor Necrosis Factor α (TNF-α) Detection Kit

Product code: YPJ1887
Promotional price: $ 458

Product introduction

Reactive species
chicken
Examination area
7.8-500pg/mL
Sensitivity
Physical properties
Each liquid component is clear and transparent, without precipitation or floccules. Microplate aluminum foil bagIt should be vacuum packed without damage or leakage.
Use
Used to detect the concentration of chicken tumor necrosis factor α (TNF-α) in samples such as serum, plasma, and cell culture supernatant.
Experimental principle
The kit uses enzyme-linked immunosorbent assay. Biotin-labeled chicken tumor necrosis factor α (TNF-α) and purified anti-chicken tumor necrosis factor α (TNF-α) antibodies are used to coat microplates. In the competitive inhibition reaction, a certain amount of solid-phase antibody and biotin-labeled chicken tumor necrosis factor α (TNF-α) and non-labeled antigen (calibrator or specimen) inhibit the competitive reaction. The binding amount of antibody and biotin-labeled chicken tumor necrosis factor α (TNF-α) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of antibody and biotin-labeled chicken tumor necrosis factor α (TNF-α), and vice versa. After the reaction is balanced, solid-phase antibody-biotinylated chicken tumor necrosis factor α (TNF-α) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated chicken tumor necrosis factor α (TNF-α)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of chicken tumor necrosis factor α (TNF-α) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of chicken tumor necrosis factor α (TNF-α) in samples.
Specificity
This kit recognizes natural and recombinant chicken tumor necrosis factor α (TNF-α) with no cross-talk with structural analogs.

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