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Leukotriene A4 (LTA4) Detection Kit

Product code: YPJ1502
Promotional price: $ 458

Product introduction

Reactive species
Common species
Examination area
123.5-10,000pg/mL
Sensitivity
Physical properties
Each liquid component is clear and transparent, without precipitation or floccules. Microplate aluminum foil bagIt should be vacuum packed without damage or leakage.
Use
Used to detect the concentration of leukotriene A4 (LTA4) in samples such as serum, plasma, and cell culture supernatant.
Experimental principle
The kit uses enzyme-linked immunosorbent assay. Biotin-labeled leukotriene A4 (LTA4) and purified anti-leukotriene A4 (LTA4) antibodies are coated on microplates. In the competitive inhibition reaction, a certain amount of solid-phase antibodies and biotin-labeled leukotriene A4 (LTA4) and non-labeled antigens (calibrators or specimens) inhibit the competitive reaction. The binding amount of the antibody and biotin-labeled leukotriene A4 (LTA4) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of the antibody and biotin-labeled leukotriene A4 (LTA4), and vice versa. After the reaction is balanced, a solid-phase antibody-biotinylated leukotriene A4 (LTA4) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated leukotriene A4 (LTA4)-enzyme-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of leukotriene A4 (LTA4) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of leukotriene A4 (LTA4) in samples.
Specificity
This kit recognizes natural and recombinant leukotriene A4 (LTA4) with no cross-talk with structural analogs.

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