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Acetyl Coenzyme A (A-CoA) Detection Kit

Product code: YPJ1151
Promotional price: $ 458

Product introduction

Reactive species
Common species
Examination area
0.625-20 ng/mL
Sensitivity
Physical properties
Each liquid component is clear and transparent, without precipitation or floccules. Microplate aluminum foil bagIt should be vacuum packed without damage or leakage.
Use
Used to detect the concentration of acetyl coenzyme A (A-CoA) in samples such as serum, plasma, and cell culture supernatant.
Experimental principle
The kit uses enzyme-linked immunosorbent assay. Biotin-labeled acetyl coenzyme A (A-CoA) is used, and the purified anti-acetyl coenzyme A (A-CoA) antibody is coated on the microplate. In the competitive inhibition reaction, a certain amount of solid-phase antibody and biotin-labeled acetyl coenzyme A (A-CoA) and non-labeled antigen (calibrator or specimen) inhibit the competitive reaction. The binding amount of the antibody and biotin-labeled acetyl coenzyme A (A-CoA) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less the antibody and biotin-labeled acetyl coenzyme A (A-CoA) bind, and vice versa. After the reaction is balanced, a solid-phase antibody-biotinylated acetyl coenzyme A (A-CoA) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated acetyl coenzyme A (A-CoA)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of acetyl coenzyme A (A-CoA) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of acetyl coenzyme A (A-CoA) in the sample.
Specificity
This kit recognizes natural and recombinant acetyl-CoA (A-CoA) with no crosstalk with structural analogs.

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