Reactive species
Human;Mouse;Rat
Protein name
Appetite-regulating hormone
Immunogen
The antiserum was produced against synthesized peptide derived from human Ghrelin. AA range:47-96
Specificity
Ghrelin Monoclonal Antibody detects endogenous levels of Ghrelin protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Monoclonal, Mouse,IgG
Dilution rate
Western Blot: 1/500 - 1/2000
Purification process
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
GHRL; MTLRP; Appetite-regulating hormone; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; Protein M46
Background
This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function
Function
function:Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.,function:Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.,mass spectrometry:Ghrelin-27-C10, O-decanoylated form PubMed:12414809,mass spectrometry:Ghrelin-27-C8, O-octanoylated form PubMed:12414809,mass spectrometry:Ghrelin-28-C10, O-decanoylated form PubMed:12414809,mass spectrometry:Ghrelin-28-C10:1, O-decenoylated form PubMed:12414809,mass spectrometry:Ghrelin-28-C8, O-octanoylated form PubMed:12414809,online information:Ghrelin entry,online information:Gut feelings - Issue 66 of January 2006,PTM:Amida
