Reactive species
Human;Rat;Mouse;
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 155-254 of human HLADQA1 (P01909)
Dilution rate
WB 1:500-2000
Purification process
Affinity purification
Stockpile
Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Other name
DQA1; DQ-A1; CELIAC1; HLA-DQA; HLA-DQB1; HLA-DQA1*; HLA-DQA1
Background
HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a
heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the
membrane. It plays a central role in the immune system by presenting peptides derived
from extracellular proteins. Class II molecules are expressed in antigen presenting cells
(APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35
kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the
two extracellular domains, and exon 4 encodes the transmembrane domain and the
cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the
polymorphisms specifying the peptide binding specificities, resulting in up to four different
molecules. Typing for these polymorphisms is routinely done for bone marrow
transplantation.
