Reactive species
Human;Rat;Mouse;
Protein name
Ubiquitin carboxyl-terminal hydrolase 1 (EC 3.4.19.12) (Deubiquitinating enzyme 1) (hUBP) (Ubiquitin thioesterase 1) (Ubiquitin-specific-processing protease 1)
Immunogen
Synthesized peptide derived from part region of human protein
Specificity
UBP1 Monoclonal Antibody detects endogenous levels of protein.
Constitute
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Source
Monoclonal, Mouse,IgG
Dilution rate
WB 1:500-2000
Purification process
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008],
Function
catalytic activity:Ubiquitin C-terminal thioester + H(2)O = ubiquitin + a thiol.,developmental stage:Cell cycle-regulated. Highest level during S phase.,function:Negative regulator of DNA damage repair. Autocatalytic cleavage of USP1 leads to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis following DNA damage. Regulates ubiquitination of FANCD2.,miscellaneous:HEK293T cells expressing reduced levels of USP1 show a higher level of ubiquitinated PCNA and an increase in point mutations upon UV irradiation.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Ubiquitinated. Degraded by the proteasome.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the peptidase C19 family.,subunit:Interacts with FANCD2.,
