Reactive species
Human;Rat;Mouse;
Gene Name
PABPC4 APP1 PABP4
Protein name
Polyadenylate-binding protein 4 (PABP-4) (Poly(A)-binding protein 4) (Activated-platelet protein 1) (APP-1) (Inducible poly(A)-binding protein) (iPABP)
Immunogen
Synthesized peptide derived from human protein . at AA range: 190-270
Specificity
PABP4 Monoclonal Antibody detects endogenous levels of protein.
Constitute
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Source
Monoclonal, Mouse,IgG
Dilution rate
WB 1:500-2000
Purification process
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Poly(A)-binding proteins (MABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. MABPC4 or IMABP (inducible MABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased MABPC4 mRNA levels in T cells approximately 5-fold. MABPC4 contains 4 RNA-binding domains and proline-rich C terminus. MABPC4 is localized primarily to the cytoplasm. It is suggested that MABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. MABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated Mouse platelets. MABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript va
Function
function:Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo.,PTM:Arg-518 is dimethylated, probably to asymmetric dimethylarginine.,similarity:Belongs to the polyadenylate-binding protein type-1 family.,similarity:Contains 1 MABC domain.,similarity:Contains 4 RRM (RNA recognition motif) domains.,subunit:Interacts with NFX1.,tissue specificity:Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated.,
