Reactive species
Human;Mouse;
Antibody type
Monoclonal antibodies
Protein name
Serine/threonine-protein kinase PAK 4
Immunogen
Synthesized peptide derived from human protein.AA range:400-500
Specificity
This antibody detects endogenous levels of PAK4.
Constitute
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Mouse, Monoclonal/IgG3, kappa
Dilution rate
WB 1:500-2000. IF 1:100-500. ELISA 1:1000-5000
Purification process
Protein G
Stockpile
-15°C to -25°C/1 year(Do not lower than -25°C)
Other name
PAK4;KIAA1142;Serine/threonine-protein kinase PAK 4;p21-activated kinase 4;PAK-4
Background
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Function
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.,PTM:Autophosphorylated on
