Reactive species
Human;Mouse;Rat;
Applications
IHC;IF;ELISA
Antibody type
Monoclonal antibodies
Immunogen
Synthesized peptide derived from human CD20 AA range: 200-297
Specificity
This antibody detects endogenous levels of CD20.
Constitute
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Mouse, Monoclonal/IgG1, kappa
Dilution rate
IHC 1:200-1000. IF 1:100-500. ELISA 1:1000-5000
Purification process
Protein G
Stockpile
-15°C to -25°C/1 year(Do not lower than -25°C)
Other name
B-lymphocyte antigen CD20 (B-lymphocyte surface antigen B1;Bp35;Leukocyte surface antigen Leu-16;Membrane-spanning 4-domains subfamily A member 1;CD antigen CD20)
Background
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008],
Function
caution:Epitope 1, mapped in PubMed:16785532, is predicted to be buried in the membrane. Its accessibility to the extracellular space, and thus to antibody recognition, is not explained.,function:This protein may be involved in the regulation of B-cell activation and proliferation.,online information:CD20 entry,pharmaceutical:Monoclonal antibodies(mAb) against CD20 are used to treat B-cell non-Hodgkin's lymphoma (NHL). These antibodies include Rituximab (Mabthera), Britumomab (Zevalin) and Tositumomab (Bexxar). CD20 engaged by mAb can generate transmembrane signals capable of directly controlling cell growth and triggering cell death in certain tumors. Alternatively, mAb
