Applications
IHC;WB;IF;ELISA
Antibody type
Monoclonal antibodies
Protein name
Arginase-1 (EC 3.5.3.1) (Liver-type arginase) (Type I arginase)
Immunogen
Synthesized peptide derived from human Arginase-1 AA range: 200-322
Specificity
The antibody can specifically recognize human Arginase-1 protein. In western blotting of HepG2 cell lysate, the antibody can label a 35 kDa band corresponding to Arginase-1.
Constitute
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Mouse, Monoclonal/IgG2b, kappa
Dilution rate
IHC 1:200-1000. WB 1:500-2000. IF 1:100-500. ELISA 1:1000-5000
Purification process
Protein G
Stockpile
-15°C to -25°C/1 year(Do not lower than -25°C)
Background
Arginase-1 is a key enzyme in the urea cycle, which can catalyze the decomposition of arginine into ornithine and urea. Highly specific expression in liver tissue, but not in bile duct epithelial cells, hepatic sinusoidal endothelial cells, Kupffer cells and vascular endothelial cells.
Function
catalytic activity:L-arginine + H(2)O = L-ornithine + urea.,cofactor:Binds 2 manganese ions per subunit.,disease:Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.,induction:By arginine or homoarginine.,online information:Arginase entry,pathway:Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.,similarity:Belongs to the arginase family.,subunit:Homotrimer.,
