Reactive species
Human;Mouse (predicted: Rat)
Antibody type
Monoclonal antibodies
Protein name
Cytokeratin-20
Immunogen
Synthesized peptide derived from human CK20 AA range:300-424
Specificity
This antibody detects endogenous levels of Cytokeratin 20
Constitute
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Monoclonal, Rabbit IgG1, Kappa
Dilution rate
IHC 1:100-500, ELISA 1:5000-20000
Purification process
Recombinant Expression and Affinity purified
Stockpile
-15°C to -25°C/1 year(Do not lower than -25°C)
Background
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa.The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008],
Function
developmental stage:First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.,function:Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,PTM:Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during t
