Reactive species
Human;Mouse;
Applications
IHC;WB;IF;ELISA
Antibody type
Monoclonal antibodies
Gene Name
CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1
Immunogen
Synthesized peptide derived from human p21 AA range: 1-100
Specificity
This antibody detects endogenous levels of p21 protein.
Constitute
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Mouse, Monoclonal/IgG1, kappa
Dilution rate
IHC 1:200-1000. WB 1:500-2000. IF 1:100-500. ELISA 1:1000-5000
Purification process
Protein G
Stockpile
-15°C to -25°C/1 year(Do not lower than -25°C)
Other name
Cyclin-dependent kinase inhibitor 1 ;CDK-interacting protein 1;Melanoma differentiation-associated protein 6;MDA-6;p21;
Function
function:May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.,induction:By p53, mezerein (antileukemic compound) and interferon beta.,PTM:Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.,similarity:Belongs to the CDI family.,tissue specificity:Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.,
