Applications
IHC;WB;IF;ELISA
Antibody type
Monoclonal antibodies
Immunogen
Synthesized peptide derived from human CD3, CD3E AA range: 1-100
Specificity
The antibody can specifically recognize human CD3e protein, and shows no reaction with CD3d or CD3g. In western blotting of Jurkat cell lysate, the antibody can label a 23 kDa band corresponding to CD
Constitute
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Mouse, Monoclonal/IgG2a, kappa
Dilution rate
IHC 1:200-1000. WB 1:500-2000. IF 1:100-500. ELISA 1:1000-5000
Purification process
Protein G
Stockpile
-15°C to -25°C/1 year(Do not lower than -25°C)
Other name
4930549J05Rik；A430104F18Rik；AW552088；Cd247；CD247 antigen；CD247 antigen, zeta subunit；CD247 molecule；CD3；CD3 antigen, delta subunit；CD3 delta；CD3 epsilon；CD3 eta；CD3 gamma；CD3 molecule delta polypeptide；CD3 molecule, epsilon polypeptide；CD3 molecule, gamma polypeptide；CD3 zeta；CD3-DELTA；CD3d；CD3D antigen delta polypeptide；CD3d antigen, delta polypeptide (TiT3 complex)；CD3d molecule delta；CD3d molecule delta CD3 TCR complex；CD3d molecule, delta (CD3-TCR complex)；CD3D_HUMAN；CD3E；CD3e antigen；CD3E antigen epsilon polypeptide；CD3e antigen, epsilon polypeptide (TiT3 complex)；CD3e molecule epsilon；CD3e molecule epsilon CD3 TCR complex；CD3e molecule, epsilon (CD3-TCR complex)；CD3epsilon；CD3G；CD3g antigen；CD3G antigen gamma polypeptide；CD3g antigen, gamma polypeptide (TiT3 complex)；CD3g molecule gamma；CD3g molecule gamma CD3 TCR complex；CD3g molecule, gamma (CD3-TCR complex)；CD3H；CD3Q；CD3Z；CD3zeta；Ctg3；FLJ17620；FLJ17664；FLJ18683；FLJ79544；FLJ94613；IMD19；Leu-4；MGC138597；OKT3, delta chain；OTTHUMP0
Background
CD3 protein contains ε、γ、δ and ζ four subunits, including extracellular, transmembrane and intracellular domains. CD3 is a T cell marker, which is not expressed by B cells. CD3 complex mediates TCR antigen recognition, leading to T cell proliferation and cytokine release. It is mainly used for the diagnosis of T-cell lymphoma, with high specificity but low sensitivity. In order to improve the accuracy of diagnosis, it is usually combined with CD45RO in clinical application.
Function
function:The CD3 complex mediates signal transduction.,online information:CD3E mutation db,similarity:Contains 1 Ig-like (immunoglobulin-like) domain.,similarity:Contains 1 ITAM domain.,subunit:The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.,
