Reactive species
Human;Mouse;Rat
Antibody type
Polyclonal Antibody
Protein name
Gasdermin domain-containing protein 1,Gasdermin-D,GSDMD,GSDMD-CT,GSDMD-NT,GSDMD_HUMAN
Immunogen
Synthesized peptide derived from human GSDMD. AA range:1-100
Specificity
The antibody detects endogenous full lenth and n-ternal fragment of gsdmd protein,
Constitute
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
IF 1:50-200 WB 1:1000-3000 IHC 1:50-300
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Stockpile
-15°C to -25°C/1 year(Do not lower than -25°C)
Function
Cleavage at Asp-275 by CASP1 (mature and uncleaved precursor forms), CASP4, CASP5 or CASP8 relieves autoinhibition and is sufficient to initiate pyroptosis (PubMed:26375003, PubMed:29898893, PubMed:32109412). Cleavage by CASP1 and CASP4 is not strictly dependent on the consensus cleavage site on GSDMD but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32109412). Cleavage by CASP8 takes place following inactivation of MAP3K7/TAK1 by Yersinia toxin YopJ (By similarity). Cleavage at Asp-87 by CASP3 or CAPS7 inactivates the ability to mediate pyroptosis (PubMed:28392147, PubMed:28045099).
