Applications
IHC;WB;ELISA
Antibody type
Monoclonal antibodies
Immunogen
Synthesized peptide derived from human CD8 a AA range: 150-235
Specificity
This antibody detects endogenous levels of CD8 protein
Constitute
PBS, pH7.4, 50% glycerol, 0.05% Proclin 300
Source
Monoclonal, Mouse IgG2b, Kappa
Dilution rate
IHC-p 1:200-400, WB 1:200-1000, ELISA 1:5000-20000
Purification process
Protein G
Stockpile
-15°C to -25°C/1 year(Do not lower than -25°C)
Other name
T-cell surface glycoprotein CD8 alpha chain (T-lymphocyte differentiation antigen T8/Leu-2;CD antigen CD8a;
Background
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011],
Function
disease:Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.,function:Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.,online information:CD8 entry,online information:CD8A mutation db,PTM:All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subunit:In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers.
