Applications
IHC;WB; ELISA
Antibody type
Monoclonal antibodies
Immunogen
Synthesized peptide derived from human CK19 AA range:300-400
Specificity
This antibody detects endogenous levels of Cytokeratin 19
Constitute
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Monoclonal, Rabbit IgG1, Kappa
Dilution rate
IHC-p 1:100-500, WB 1:500-1000, ELISA 1:5000-20000
Purification process
Recombinant Expression and Affinity purified
Stockpile
-15°C to -25°C/1 year(Do not lower than -25°C)
Other name
40 kDa keRatin intermediate filament;CK 19;CK-19;CK19;Cytokeratin 19;CytokeRatin-19;K19;K1C19_HUMAN;K1CS;KeRatin 19;KeRatin type I 40 kD;KeRatin type I 40kD;KeRatin type I cytoskeletal 19;KeRatin, type I cytoskeletal 19;KeRatin, type I, 40 kd;KeRatin-19;KRT19;MGC15366
Background
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008],
Function
developmental stage:Present in hair follicles at all stages of development.,domain:This keratin differs from all other IF proteins in lacking the C-terminal tail domain.,function:Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. Interacts with PNN and the actin-binding domain of DMD. Interacts with HCV core protein.,tissue specificity:Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestin
