Antibody type
Monoclonal antibodies
Protein name
Epidermal growth factor receptor
Immunogen
Synthetic Peptide of EGFR AA range: 300-400
Specificity
The antibody detects endogenous EGFR proteins.
Constitute
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Source
Monoclonal, Mouse IgG2a, Kappa
Dilution rate
WB 1:1000,ELISA 1:5000-20000
Purification process
The antibody was affinity-purified from mouse ascites by affinity- chromatography using specific immunogen.
Stockpile
-15°C to -25°C/1 year(Do not lower than -25°C)
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016],
Function
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Defects in EGFR are associated with lung cancer [MIM:211980].,function:Isoform 2/truncated isoform may act as an antagonist.,function:Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta- catenin.,miscellaneous:Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.,online information:EGFR entry,PTM:Monoubiquitinated and polyubiquitinated upon EGF stimu
