Applications
WB,IHC-F,IHC-P,FC,ICC/IF,IP
Antibody type
Monoclonal antibodies
Dalton（DA）
Calculated MW: 75 kDa,Observed MW:75 kDa
Immunogen
synthetic phospho-peptide corresponding to residues surrounding serine 166 of Mouse RIPK1
Dilution rate
WB: 1/1000 - 1/2000; IP: 1/50 - 1/500; ICC/IF: 1/100 - 1/500; IHC-P: 1/100 - 1/500; IHC-F: 1/100 - 1/500
Stockpile
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Other name
Cell death protein RIP,Receptor-interacting protein 1
Background
Receptor-interacting serine/threonine-protein kinase 1 is a serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. RIPK1 is phosphorylated at several sites within the kinase domain that are sensitive to Nec-1, including Ser14, Ser15, Ser161, and Ser166.
