Reactive species
Human,Mouse,Rat
Antibody type
Monoclonal antibodies
Dalton（DA）
Calculated MW: 25 kDa; Observed MW: 25 kDa
Immunogen
synthetic peptide of human eIF4E
Dilution rate
WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
Stockpile
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Other name
EIF4E; EIF4EL1;EIF4F; Eukaryotictranslation initiation factor 4E; elF-4E; elF4EelF-4F 25 kDa subunit; mRNA cap-bindingprotein
Background
eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
