Reactive species
Human;Mouse
Antibody type
Polyclonal Antibody
Protein name
Centromere-associated protein E (Centromere protein E) (CENP-E) (Kinesin-related protein CENPE)
Immunogen
Synthesized peptide derived from human CENPE
Specificity
This antibody detects endogenous levels of CENPE at Human, Mouse
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Stockpile
-15°C to -25°C/1 year(Do not lower than -25°C)
Function
Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules . The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated . Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends . Suppresses chromosome c
