Applications
WB,IHC-P,ICC/IF,FC
Antibody type
Monoclonal antibodies
Dalton（DA）
Calculated MW: 42,44 kDa; Observed MW: 42,44 kDa
Immunogen
synthesized peptide derived from human Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187)
Dilution rate
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100
Stockpile
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Background
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements.
