Reactive species
Human,Mouse,Rat
Applications
WB,ICC/IF,IHC-P
Antibody type
Polyclonal Antibody
Dalton（DA）
Calculated MW: 61 kDa; Observed MW: 61 kDa
Immunogen
The antiserum was produced against synthesized peptide derived from human Chk2 around the phosphorylation site of Thr68.
Dilution rate
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Stockpile
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Other name
CHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2
Background
These are known to be preferred sites for phosphorylation by ATM/ATR kinases. After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function.
