Reactive species
Human,Mouse,Rat
Applications
WB,IHC-F,IHC-P,ICC/IF,IP
Antibody type
Monoclonal antibodies
Dalton（DA）
Calculated MW: 15 kDa; Observed MW: 15 kDa
Immunogen
synthetic peptide of human Histone H2A.X
Dilution rate
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Stockpile
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Other name
H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X
Background
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
