Reactive species
Human,Mouse,Rat
Applications
WB,IHC-P,ICC/IF
Antibody type
Polyclonal Antibody
Dalton（DA）
Calculated MW: 15 kDa; Observed MW: 15 kDa
Immunogen
Peptide sequence around Tri-Methylation site of lysine 27(A-R-K(tri-methyl)-S-A) derived from Human Histone H3.
Dilution rate
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Stockpile
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Other name
H3K27me3; H3 histone; HIST1H3A; Histone cluster 1; H3a
Background
H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
