Reactive species
Human; Mouse; Rat
Antibody type
Monoclonal antibodies
Protein name
Mitogen-activated protein kinase 3
Immunogen
Synthesized peptide derived from human protein. AA range: 150-250
Specificity
This antibody detects endogenous levels of ERK 1/2 at Human, mouse, rat
Constitute
Liquid in PBS containing 50% glycerol, and 0.05% proclin 300.
Source
Monoclonal, Mouse lgG1,kappa
Dilution rate
WB 1:500-2000
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Stockpile
‘-15°C to -25°C/1 year(Do not lower than -25°C)
Background
mitogen-activated protein kinase 3(MAPK3) Homo sapiens The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008],
Function
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon
