Reactive species
Human,Rat,Mouse
Antibody type
Monoclonal antibodies
Immunogen
Purified recombinant protein expressed in E.coli.
Dilution rate
IHC: 1/50-1/100
Stockpile
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Other name
hAGP7; Ubiquitin-activating enzyme E1-like protein; APG7L
Background
The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.
