Reactive species
Human,Mouse,Rat
Applications
WB,IHC-P,ICC/IF
Antibody type
Polyclonal Antibody
Dalton（DA）
Calculated MW: 60 kDa; Observed MW: 65 kDa
Immunogen
Peptide sequence around phosphorylation site of serine 536 (F-S-S(p)-I-A) derived from Human NFκB-p65.
Dilution rate
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Stockpile
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Other name
NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB
Background
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.
