Reactive species
Human;Mouse;Rat
Antibody type
Polyclonal Antibody
Protein name
Insulin-induced gene 1 protein (INSIG-1)
Immunogen
Synthesized peptide derived from human INSIG1
Specificity
This antibody detects endogenous levels of INSIG1 at Human, Mouse,Rat
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
WB 1:500-2000
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Stockpile
‘-15°C to -25°C/1 year(Do not lower than -25°C)
Function
Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of SCAP and degradation of HMGCR . Acts as a negative regulator of cholesterol biosynthesis by mediating the retention of the SCAP-SREBP complex in the endoplasmic reticulum, thereby blocking the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 . Binds oxysterol, including 25-hydroxycholesterol, regulating interaction with SCAP and retention of the SCAP-SREBP complex in the endoplasmic reticulum . In presence of oxysterol, interacts with SCAP, retaining the SCAP-SREBP complex in the endoplasmic reticulum, thereby preventing SCAP from escorting SREBF1/SREBP1 and SREBF2/SREBP2 to the Golgi . Sterol deprivation or phosphorylation by PCK1 reduce oxysterol-binding, disrupting the interaction between INS
