Reactive species
Human,Mouse
Applications
WB,IHC-P,ICC/IF,FC
Antibody type
Monoclonal antibodies
Dalton（DA）
Calculated MW: 60 kDa; Observed MW: 65 kDa
Immunogen
Purified recombinant fragment of human SMAD4 expressed in E. Coli.
Dilution rate
WB: 1/500-1/1000 IHC: 1/100-1/200 IF: 1/50-1/200 FC: 1/50-1/100
Stockpile
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Other name
SMAD4; DPC4; MADH4; Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4
Background
Smad4 transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.
