Reactive species
Human,Mouse,Rat
Applications
WB,IHC-F,IHC-P,ICC/IF
Cited
WB (Chicken)
Dalton（DA）
Calculated MW: 42 kDa; Observed MW: 42 kDa
Immunogen
A synthetic peptide of human beta Actin
Dilution rate
WB: 1/5000-1/10000 IHC: 1/50-1/200 IF: 1/100-1/200
Stockpile
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Other name
ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta
Background
beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
