Reactive species
Human,Mouse,Rat
Applications
WB,IHC-P,ICC/IF,IP,FC
Antibody type
Monoclonal antibodies
Dalton（DA）
Calculated MW: 56 kDa
Immunogen
synthesized peptide derived from human AKT1
Dilution rate
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/50 FC: 1/50-1/100
Stockpile
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Background
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
