Reactive species
Human;Mouse
Antibody type
Polyclonal Antibody
Protein name
Putative methyltransferase NSUN6 (EC 2.1.1.-) (NOL1P2/Sun and PUA domain-containing protein 1) (NOL1P2/Sun domain family member 6)
Immunogen
Synthesized peptide derived from human NSUN6
Specificity
This antibody detects endogenous levels of NSUN6 at Human, Mouse
Constitute
Liquid in PBS containing 50% glycerol, and 0.600% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
WB 1:500-2000
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Stockpile
‘-15°C to -25°C/1 year(Do not lower than -25°C)
Function
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA) . In vitro also methylates tRNA(Thr)(AGT) . Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates .
