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Vinculin mouse mAb(PT0372)

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Vinculin mouse mAb(PT0372)

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Product code: YP-Ab-17654

Promotional price: $ 255

100UL 53UL 10UL

Product introduction

Reactive species

Human;Mouse;Rat

Applications

IHC;IF;ELISA

Antibody type

Monoclonal antibodies

Gene Name

VCL

Protein name

Vinculin

Dalton（DA）

123kD

Immunogen

Synthesized peptide derived from human Vinculin. AA range:786-835

Specificity

Vinculin Antibody detects endogenous levels of Vinculin protein.

Constitute

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Monoclonal, Mouse,IgG1

Dilution rate

Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.

Purification process

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

mg/ml

Stockpile

-20°C/1 year

Other name

VCL; Vinculin; Metavinculin

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008],

Function

disease:Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) [MIM:611407]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.,function:Involved in cell adhesion. May be involved in the attachment of the actin-based microfilaments to the plasma membrane. May also play important roles in cell morphology and locomotion.,online information:Vinculin entry,PTM:Aceylated; mainly by myristic acid but also small amount of palmitic acid.,PTM:Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques.,similarity:Belongs to the vinculin/alpha-catenin family.,s

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IP

常规Immunoprecipitation（免疫沉淀 IP）实验操作步骤

WB

蛋白免疫印迹（WB) 实验步骤

Vinculin mouse mAb(PT0372)

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Vinculin mouse mAb(PT0372)

Product introduction

Product Details

Customer data and reviews (0)

Citation

Background information

FAQ

Experimental scheme

IP

WB

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