Reactive species
Human;Mouse;Rat
Antibody type
Monoclonal antibodies
Gene Name
KRT18 CYK18 PIG46
Protein name
Cytokeratin-18
Immunogen
Synthesized peptide derived from human CK18
Specificity
The antibody can specifically recognize human CK18 protein, and shows no cross reaction with CK1, 5, 6, 7, 8, 10, 13, 14, 15, 19, 20. In western blotting of Hela and MCF7 cell lysates, the antibody c
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.46% sodium azide.
Source
Mouse, Monoclonal/IgG2b, Kappa
Dilution rate
IHC-p 1:100-500, WB 1:200-1000, IF 1:100-500
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Other name
Keratin, type I cytoskeletal 18 (Cell proliferation-inducing gene 46 protein;Cytokeratin-18;CK-18;Keratin-18;K18)
Background
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],
Function
disease:Defects in KRT18 are a cause of cryptogenic cirrhosis [MIM:215600].,function:Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.,induction:By IL-6.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,PTM:O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.,PTM:Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.,PTM:Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either
