Reactive species
Species independent
Applications
WB;ELISA;IP;IF;CoIP
Gene Name
Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
Immunogen
Synthetic Peptide of Flag-Tag
Specificity
The antibody detects C-terminal, internal, and N-terminal Flag-tag fusion proteins.
Constitute
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Source
Mouse VH IgG1 and VL Kappa was expressed from 293F cells
Dilution rate
WB: 1:10000-50000 ELISA 1:20000-50000 IP:100-1:200 IF: 1:1000 -3000
Purification process
Protein A purified
Background
The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
