Reactive species
Human;Mouse;Rat
Applications
IHC;IF;WB;ELISA
Antibody type
Polyclonal Antibody
Immunogen
The antiserum was produced against synthesized peptide derived from human Claudin 5 around the phosphorylation site of Tyr217. AA range:169-218
Specificity
Phospho-Claudin-5 (Y217) Polyclonal Antibody detects endogenous levels of Claudin-5 protein only when phosphorylated at Y217.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
WB 1:500-2000 IHC: 1/100 - 1/300. ELISA: 1/5000.. IF 1:50-200
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
CLDN5; AWAL; TMVCF; Claudin-5; Transmembrane protein deleted in VCFS; TMDVCF
Background
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008],
Function
function:Plays a major role in tight junction-specific obliteration of the intercellular space.,similarity:Belongs to the claudin family.,subunit:Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ.,
