Reactive species
Human;Mouse;Rat
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
Mitotic checkpoint protein BUB3
Immunogen
The antiserum was produced against synthesized peptide derived from human BUB3. AA range:279-328
Specificity
Bub3 Polyclonal Antibody detects endogenous levels of Bub3 protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
BUB3; Mitotic checkpoint protein BUB3
Background
This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],
Function
function:Required for kinetochore localization of BUB1.,similarity:Belongs to the WD repeat BUB3 family.,similarity:Contains 5 WD repeats.,subunit:Interacts with BUB1 and BUBR1.,
