Reactive species
Human;Mouse
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
Cell division cycle protein 16 homolog
Immunogen
The antiserum was produced against synthesized peptide derived from human CDC16/APC6 around the phosphorylation site of Ser560. AA range:526-575
Specificity
Phospho-Cdc16 (S560) Polyclonal Antibody detects endogenous levels of Cdc16 protein only when phosphorylated at S560.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
CDC16; ANAPC6; Cell division cycle protein 16 homolog; Anaphase-promoting complex subunit 6; APC6; CDC16 homolog; CDC16Hs; Cyclosome subunit 6
Background
The protein encoded by this gene functions as a protein ubiquitin ligase and is a component of the multiprotein APC complex. The APC complex is a cyclin degradation system that governs exit from mitosis by targeting cell cycle proteins for degredation by the 26S proteasome. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein, and other APC complex proteins, contain a tetratricopeptide repeat (TPR) domain; a protein domain that is often involved in protein-protein interactions and the assembly of multiprotein complexes. Multiple alternatively spliced transcript variants, encoding distinct proteins, have been identified. [provided by RefSeq, Jan 2016],
Function
function:Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.,similarity:Belongs to the APC6/CDC16 family.,similarity:Contains 7 TPR repeats.,subcellular location:Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.,subunit:The APC/C is composed of at least 11 subunits. Interacts with PPP5C and CDC20.,
