Reactive species
Human;Mouse;Rat;Monkey
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
Tumor susceptibility gene 101 protein
Immunogen
The antiserum was produced against synthesized peptide derived from human TSG101. AA range:281-330
Specificity
Tsg 101 Polyclonal Antibody detects endogenous levels of Tsg 101 protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
TSG101; Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101
Background
The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008],
Function
alternative products:Additional isoforms seem to exist. Several shorter isoforms are detected in primary breast cancers and other tumors,domain:The coiled coil domain may interact with stathmin.,domain:The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.,domain:The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.,function:Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a
