Reactive species
Human;Mouse;Rat;Monkey
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
Rac GTPase-activating protein 1
Immunogen
The antiserum was produced against synthesized peptide derived from human GTPase Activating Protein. AA range:353-402
Specificity
Rac GAP1 Polyclonal Antibody detects endogenous levels of Rac GAP1 protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
RACGAP1; KIAA1478; MGCRACGAP; Rac GTPase-activating protein 1; Male germ cell RacGap; MgcRacGAP; Protein CYK4 homolg; CYK4; HsCYK-4
Background
This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016],
Function
domain:The coiled coil region is indispensible for localization to the midbody during cytokinesis.,function:Essential for the early stages of embryogenesis and may play a role in the microtubule-dependent steps in cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Required for initiation of cleavage furrow ingression by regulating ECT2 and for assembly of the contractile ring. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate tra
