Antibody type
Monoclonal antibodies
Protein name
Ras association domain-containing protein 1
Immunogen
Purified recombinant human RASSF1 protein fragments expressed in E.coli.
Specificity
RASSF1 Monoclonal Antibody detects endogenous levels of RASSF1 protein.
Constitute
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Dilution rate
Western Blot: 1/1000 - 1/2000. Not yet tested in other applications.
Purification process
Affinity purification
Other name
RASSF1; RDA32; Ras association domain-containing protein 1
Background
This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, May 2011],
Function
function:Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK4 during Fas-induced apoptosis. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 Ras-associating domain.,similarity:Contains 1 SARAH domain.,subcellular location:Localizes to cytoplasmic mic
