Antibody type
Monoclonal antibodies
Protein name
POU domain class 5 transcription factor 1
Immunogen
Purified recombinant human Oct-3/4 protein fragments expressed in E.coli.
Specificity
Oct-3/4 Monoclonal Antibody detects endogenous levels of Oct-3/4 protein.
Constitute
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Dilution rate
Western Blot: 1/1000 - 1/2000. Flow cytometry: 1/100 - 1/200. Immunofluorescence: 1/100 - 1/500. Not yet tested in other applications.
Purification process
Affinity purification
Other name
POU5F1; OCT3; OCT4; OTF3; POU domain; class 5, transcription factor 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3
Background
This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013],
Function
function:Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.,miscellaneous:Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.,online information:Oct-4 entry,PTM:Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.,similarity:Belongs to the POU transcription factor family. Class-5 subfamily.,similarity:Contains 1 homeobox DNA-binding doma
