Applications
IHC,WB,ICC/IF
Antibody type
Monoclonal antibodies
Immunogen
Synthesized peptide derived from human CD3, CD3E
Specificity
The antibody can specifically recognize human CD3e protein, and shows no reaction with CD3d or CD3g. In western blotting of Jurkat cell lysate, the antibody can label a 23KDa band corresponding to CD
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.60% sodium azide.
Source
Mouse, Monoclonal/IgG2a, Kappa
Dilution rate
IHC-p 1:100-500, WB 1:200-1000, IF 1:100-500
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Other name
T-cell surface glycoprotein CD3 epsilon chain (T-cell surface antigen T3/Leu-4 epsilon chain;CD antigen CD3e)
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008],
Function
function:The CD3 complex mediates signal transduction.,online information:CD3E mutation db,similarity:Contains 1 Ig-like (immunoglobulin-like) domain.,similarity:Contains 1 ITAM domain.,subunit:The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.,
