Reactive species
Human; Predict react with Mouse, Rat
Applications
WB,IHC;IF,ELISA
Antibody type
Monoclonal antibodies
Protein name
Antigen NY-CO-13;BCC7;Cellular tumor antigen p53;FLJ92943;LFS1;Mutant tumor protein 53;p53;p53 tumor suppressor;P53_HUMAN;Phosphoprotein p53;Tp53;Transformation related protein 53;TRP53;tumor antigen
Immunogen
Synthesized peptide derived from human P53
Specificity
The antibody can recognize human wild type and mutant P53 protein. In western blotting of wild type HEK293 cell lysate, the antibody can label a 50 kDa band corresponding to P53, while there is no ban
Constitute
PBS, pH7.2, 0.03% Porcolin 300, containing stabilizing protein
Source
Monoclonal Mouse IgG2a, Kappa
Dilution rate
WB 1:500-2000 ,IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Other name
Antigen NY-CO-13;BCC7;Cellular tumor antigen p53;FLJ92943;LFS1;Mutant tumor protein 53;p53;p53 tumor suppressor;P53_HUMAN;Phosphoprotein p53;Tp53;Transformation related protein 53;TRP53;tumor antigen p55;Tumor protein 53;Tumor protein p53;Tumor suppressor p53
Background
tumor protein p53(TP53) Homo sapiens This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013],
Function
cofactor:Binds 1 zinc ion per subunit.,disease:Defects in TP53 are a cause of choroid plexus papilloma [MIM:260500]. Choroid plexus papilloma is a slow-growing benign tumor of the choroid plexus that often invades the leptomeninges. In children it is usually in a lateral ventricle but in adults it is more often in the fourth ventricle. Hydrocephalus is common, either from obstruction or from tumor secretion of cerebrospinal fluid. If it undergoes malignant transformation it is called a choroid plexus carcinoma. Primary choroid plexus tumors are rare and usually occur in early childhood.,disease:Defects in TP53 are a cause of Li-Fraumeni syndrome (LFS) [MIM:151623]. LFS is an autosomal dominant familial cancer syndrome that in its classic form is defined by the existence of a proband affected by a sarcoma before 45 years with a first degree relative affected by any tumor before 45 years a
