Applications
IHC-p;IF(paraffin section)
Antibody type
Monoclonal antibodies
Protein name
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PI3-kinase subunit alpha) (PI3K-alpha) (PI3Kalpha) (PtdIns-3-kinase subunit alpha) (EC 2.7.1.153) (Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha) (PtdIns-3-kinase subunit p110-alpha) (p110alpha) (Phosphoinositide-3-kinase catalytic alpha polypeptide) (Serine/threonine protein kinase PIK3CA) (EC 2.7.11.1)
Immunogen
Synthesized peptide derived from human PIK3CA H1047R
Specificity
This antibody detects endogenous levels of human PIK3CA H1047R. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Monoclonal, Mouse/IgG1, Kappa
Dilution rate
IHC-p 1:100-500,WB 1:500-2000
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.