Reactive species
Human; Predict react with Mouse, Rat
Antibody type
Monoclonal antibodies
Gene Name
TPSAB1 TPS1 TPS2 TPSB1
Protein name
Tryptase alpha/beta-1 (Tryptase-1) (EC 3.4.21.59) (Tryptase I) (Tryptase alpha-1)
Immunogen
Synthesized peptide derived from human Mast cell tryptase
Specificity
This antibody detects endogenous levels of human Mast cell tryptase. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Mouse, Monoclonal/IgG1, Kappa
Dilution rate
WB 500-2000 IHC-p 1:100-500. IF 1:50-200
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate gene
Function
catalytic activity:Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.,function:Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.,function:Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection.,polymorphism:There are two alleles; beta-II and beta-III which differ by 3 residues.,similarity:Belongs to the peptidase S1 family.,similarity:Belongs to the peptidase S1 family. Tryptase subfamily.,similarity:Contains 1 peptidase S1 domain.,subcellular location:Released from the secretory granules upon mast cell activation.,subunit:Homotetramer.,
