Applications
IHC-p;IF(paraffin section)
Antibody type
Monoclonal antibodies
Gene Name
PMEL D12S53E PMEL17 SILV
Protein name
Melanocyte protein PMEL (ME20-M) (ME20M) (Melanocyte protein Pmel 17) (Melanocytes lineage-specific antigen GP100) (Melanoma-associated ME20 antigen) (P1) (P100) (Premelanosome protein) (Silver locus protein homolog) [Cleaved into: M-alpha (95 kDa melanocyte-specific secreted glycoprotein) (P26) (Secreted melanoma-associated ME20 antigen) (ME20-S) (ME20S); M-beta]
Immunogen
Synthesized peptide derived from human PMEL
Specificity
This antibody detects endogenous levels of human PMEL. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Monoclonal, Mouse/IgG2b, Kappa
Dilution rate
IHC-p 1:100-500
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.