Applications
IHC-p;IF/ICC
Antibody type
Monoclonal antibodies
Protein name
DNA topoisomerase 2-alpha (EC 5.99.1.3) (DNA topoisomerase II, alpha isozyme)
Immunogen
Synthesized peptide derived from human Topoisomerase IIα
Specificity
This antibody detects endogenous levels of human Topoisomerase IIα. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Monoclonal, Mouse/IgG2b, Kappa
Dilution rate
IHC-p 1:100-500，WB 1:500-2000，IF 1:500-200
Purification process
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
