Reactive species
Human;Mouse;Rat
Antibody type
Polyclonal Antibody
Protein name
Alkaline phosphatase tissue-nonspecific isozyme
Immunogen
The antiserum was produced against synthesized peptide derived from human ALPL. AA range:201-250
Specificity
TNAP Polyclonal Antibody detects endogenous levels of TNAP protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
ALPL; Alkaline phosphatase; tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme
Background
This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [prov
Function
catalytic activity:A phosphate monoester + H(2)O = an alcohol + phosphate.,cofactor:Binds 1 magnesium ion.,cofactor:Binds 2 zinc ions.,disease:Defects in ALPL are a cause of hypophosphatasia adult type (hypophosphatasia) [MIM:146300].,disease:Defects in ALPL are a cause of hypophosphatasia childhood (hypophosphatasia) [MIM:241510].,disease:Defects in ALPL are a cause of hypophosphatasia infantile (hypophosphatasia) [MIM:241500]; an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto).,function:This isozyme may play a role in skeletal mi
