Reactive species
Human;Rat;Mouse;
Antibody type
Polyclonal Antibody
Protein name
Phosphorylase b kinase gamma catalytic chain skeletal muscle isoform
Immunogen
The antiserum was produced against synthesized peptide derived from human PHKG1. AA range:241-290
Specificity
PHKG1 Polyclonal Antibody detects endogenous levels of PHKG1 protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
WB 1:500-2000;IHC-p 1:50-300
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
PHKG1; PHKG; Phosphorylase b kinase gamma catalytic chain; skeletal muscle isoform; Phosphorylase kinase subunit gamma-1
Background
This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012],
Function
catalytic activity:2 ATP + phosphorylase b = 2 ADP + phosphorylase a.,domain:The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains.,function:Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Polymer of 16 chains, four each of alpha, beta, gamma, and delta. Alpha and beta are regulatory chains, gamma is the catalytic chain, and delta is calmodulin.,
